F54513

antibody from NSJ Bioreagents

Targeting: HSD17B10 17b-HSD10, ABAD, CAMR, ERAB, HADH2, MHBD, MRPP2, MRXS10, SDR5C1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54513

Provider

NSJ Bioreagents

Product name

HSD17B10 Antibody / ERAB

Antibody type

Polyclonal

Description

This highly specific HSD17B10 antibody is suitable for use in Western blot/Flow cytometry/Immunohistochemistry/Immunofluorescence applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.05 ml, 0.2 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the HSD17B10 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) Jurkat, 3) K562, 4) HepG2, 5) SH-SY5Y, 6) rat brain and 7) mouse brain lysate with HSD17B10 antibody. Predicted molecular weight ~27 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of fixed and permeabilized human HeLa cells with HSD17B10 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human thyroid carcinoma tissue with HSD17B10 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human HeLa cells with HSD17B10 antibody; Blue=isotype control, Green= HSD17B10 antibody.