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antibody from Invitrogen Antibodies
Targeting: HSD17B10
17b-HSD10, ABAD, CAMR, ERAB, HADH2, MHBD, MRPP2, MRXS10, SDR5C1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- PA5-27155 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ERAB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HeLaS3, Molt-4, Neuro2A, GL261, PC-12. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERAB Polyclonal Antibody (Product # PA5-27155). Sample (30 µg of whole cell lysate). A: HeLa S3. Lane B: MOLT4.15% SDS PAGE. ERAB Polyclonal Antibody (Product # PA5-27155) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERAB Polyclonal Antibody (Product # PA5-27155). Sample (30 µg of whole cell lysate). A: Neuro 2A. Lane B: GL261. 12% SDS PAGE . ERAB Polyclonal Antibody (Product # PA5-27155) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ERAB Polyclonal Antibody (Product # PA5-27155). Sample (30 µg of whole cell lysate). A: PC-12. 12% SDS PAGE. ERAB Polyclonal Antibody (Product # PA5-27155) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ERAB was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: ERAB Polyclonal Antibody (Product # PA5-27155) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERAB Polyclonal Antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ERAB Polyclonal Antibody (Product # PA5-27155) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERAB Polyclonal Antibody detects ERAB protein at mitochondria on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. ERAB Polyclonal Antibody (Product # PA5-27155) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ERAB Polyclonal Antibody detects ERAB protein at mitochondria on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ERAB Polyclonal Antibody (Product # PA5-27155) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ERAB (Product # PA5-27155) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
