Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-17887 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-MEK3/MEK6 (Ser189, Ser207) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 40 µg/mL
- Storage
- -20°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot was performed using Anti-Phospho-MEK3/MEK6 (Ser189, Ser207) Polyclonal Antibody(Product # PA5-17887) and a 39 kDa and 37 kDa bands corresponding to Phospho-MEK3/MEK6 (Ser189, Ser207) were observed in A549 cell line treated with Anisomycin and not treated with PMA. Whole Cell Extract-WCL (30 µg lysate) of A549 (Lane 1), A549 treated with 25 µg/ml Anisomycin for 30 minutes (Lane 2) and A549 treated with 200 nM PMA/TPA for 20 minutes (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).