MAB2514

antibody from Novus Biologicals

Targeting: MAP2K6 MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2514

Provider

Novus Biologicals

Product name

Mouse Monoclonal MKK3/MKK6 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MKK3/MKK6 in direct ELISAs and recombinant and endogenous MKK3/MKK6 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

The myeloperoxidase-derived oxidant HOSCN inhibits protein tyrosine phosphatases and modulates cell signalling via the mitogen-activated protein kinase (MAPK) pathway in macrophages.

Lane AE, Tan JT, Hawkins CL, Heather AK, Davies MJ
The Biochemical journal 2010 Aug 15;430(1):161-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat MKK3/MKK6 by Western Blot. Western blot shows lysates of NRK rat normal kidney cell and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3/MKK6 Monoclonal Antibody (Catalog # MAB2514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. Specific bands for MKK3 and MKK6 was detected at approximately 40 kDa and 38 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.