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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28572 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MEK6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, HeLa, HepG2, Molt-4. Predicted reactivity: Mouse (97%), Rat (97%), Zebrafish (88%), Xenopus laevis (93%), Rhesus Monkey (100%), Bovine (98%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MEK6 Polyclonal Antibody (Product # PA5-28572). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. MEK6 Polyclonal Antibody (Product # PA5-28572) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MEK6/MAP2K6 in methanol-fixed HeLa cells using a MEK6/MAP2K6 polyclonal antibody (Product # PA5-28572) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of MEK6 was performed in paraffin-embedded mouse duodenum tissue using MEK6 Polyclonal Antibody (Product # PA5-28572) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded N87 xenograft, using MEK6 (Product # PA5-28572) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
