AF1604

antibody from R&D Systems

Targeting: MAP2K6 MAPKK6, MEK6, MKK6, PRKMK6, SAPKK3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1604

Provider

R&D Systems

Product name

Human/Mouse/Rat MKK6 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human, mouse, and rat MKK6 in Western blots.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AAC50388


Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse/Rat MKK6 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, TS1 mouse helper T cell line, and B16-F1 mouse melanoma cell line. PVDF membrane was probed with 0.05 µg/mL Rabbit Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1604) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, recombinant human MKK6 (2 ng) was included. A specific band for MKK6 was detected at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Western Blot Shows Human MKK6 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and MKK6 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Mouse/Rat MKK6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1604) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MKK6 at approximately 39 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.