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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- GTX102333 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102333, RRID:AB_11163446
- Product name
- RelB antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references A high-throughput pipeline for validation of antibodies.
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F
Nature methods 2018 Nov;15(11):909-912
Nature methods 2018 Nov;15(11):909-912
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 7.5% SDS PAGE GTX102333 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Jurkat 7.5% SDS PAGE GTX102333 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (GTX102333) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RelB antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 £gg of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 £gg of RelB antibody (GTX102333) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB antibody (GTX102333) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- RelB antibody detects RelB protein at cytoplasm on human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RelB antibody (GTX102333) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 £gg/ml for 3 h) and 5 £gg of either normal rabbit IgG or anti-RelB antibody. The precipitated DNA was detected by PCR with primer set targeting to IL-1£]promoter.
