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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunohistochemistry [1]
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- Product number
- PA5-36837 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RelB (Ser552) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 70 kDa.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RelB pSer552 using Phospho-RelB pSer552 polyclonal antibody (Product # PA5-36837) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Mouse stomach tissue lysate, Lane 3: Mouse colon tissuel lysate, Lane 4: Rat colon tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RelB pSer552 using Phospho-RelB pSer552 polyclonal antibody (Product # PA5-36837) at a dilution of 1:500. Lane 1: Hela cell lysate treated with TNF-alpha, Lane 2: Mouse stomach tissue lysate, Lane 3: Mouse colon tissuel lysate, Lane 4: Rat colon tissue lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RelB (Ser552) in Lane 1: PC3 whole cell lysate (40 µg), Lane 2: PC3 treated with PMA (40 nM, 3min) whole cell lysate (40 µg), Lane 3: PC3 treated with PMA (40 nM, 15min) whole cell lysate (40 µg), Lane 4: PC3 treated with PMA. Samples were incubated with Phospho-RelB (Ser552) polyclonal antibody (Product # PA5-36837) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RelB (Ser552) in Lane 1: HeLa cell lysate treated with TNFa, Lane 2: mouse stomach tissue lysate, Lane 3: mouse colon tissue lysate, Lane 4: rat colon tissue lysate. Samples were incubated with Phospho-RelB (Ser552) polyclonal antibody (Product # PA5-36837) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RelB (Ser552) in Lane 1: PC3 whole cell lysate (40 µg), Lane 2: PC3 treated with PMA (1 nM, 3min) whole cell lysate (40 µg), Lane 3: PC3 treated with PMA (1 nM, 5min) whole cell lysate (40 µg), Lane 4: PC3 treated with PMA (1 nM, 15min) whole cell lysate (40 µg), Lane 5: PC3 treated with PMA (40 nM, 15min) whole cell lysate (40 µg). Samples were incubated with Phospho-RelB (Ser552) polyclonal antibody (Product # PA5-36837) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Phospho-RelB (Ser552) in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with Phospho-RelB (Ser552) polyclonal antibody (Product # PA5-36837) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.
