H00005971-D01P

antibody from Abnova Corporation

Targeting: RELB REL-B

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005971-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005971-D01P, RRID:AB_1712826

Product name

RELB purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RELB protein.

Antigen sequence

MLRSGPASGPSVPTGRAMPSRRVARPPAAPELGAL
GSPDLSSLSLAVSRSTDELEIIDEYIKENGFGLDG
GQPGPGEGLPRLVSRGAASLSTVTLGPVAPPATPP
PWGCPLGRLVSPAPGPGPQPHLVITEQPKQRGMRF
RYECEGRSAGSILGESSTEASKTLPAIELRDCGGL
REVEVTACLVWKDWPHRVHPHSLVGKDCTDGICRV
RLRPHVSPRHSFNNLGIQCVRKKEIEAAIERKIQL
GIDPYNAGSLKNHQEVDMNVVRICFQASYRDQQGQ
MRRMDPVLSEPVYDKKSTNTSELRICRINKESGPC
TGGEELYLLCDKVQKEDISVVFSRASWEGRADFSQ
ADVHRQIAIVFKTPPYEDLEIVEPVTVNVFLQRLT
DGVCSEPLPFTYLPRDHDSYGVDKKRKRGMPDVLG
ELNSSDPHGIESKRRKKKPAILDHFLPNHGSGPFL
PPSALLPDPDFFSGTVSLPGLEPPGGPDLLDDGFA
YDPTAPTLFTMLDLLPPAPPHASAVVCSGGAGAVV
GETPGPEPLTLDSYQAPGPGDGGTASLVGSNMFPN
HYREAAFGGGLLSPGPEAT


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RELB expression in transfected 293T cell line (H00005971-T02) by RELB MaxPab polyclonal antibody.Lane 1: RELB transfected lysate(62.10 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RELB and RELA. HeLa cells were stained with anti-RELB rabbit purified polyclonal 1:1200 and anti-RELA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)