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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
- Chromatin Immunoprecipitation [1]
- Other assay [1]
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- Product number
- PA5-27679 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RelB Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, NCI-H929, NIH-3T3, RelB-transfected 293T.
- Concentration
- 0.42 mg/mL
Submitted references Two-layer regulation of TRAF6 mediated by both TLR4/NF-kB signaling and miR-589-5p increases proinflammatory cytokines in the pathology of severe acute pancreatitis.
Chen Z, Dong WH, Wu Q, Wang J
American journal of translational research 2020;12(6):2379-2395
American journal of translational research 2020;12(6):2379-2395
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Rel B using 30 µg of Jurkat lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Rel B polyclonal antibody (Product # PA5-27679) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RelB was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RelB Polyclonal Antibody (Product # PA5-27679) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RelB was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RelB Polyclonal Antibody (Product # PA5-27679) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-RelB Polyclonal Antibody (Product # PA5-27679) and a 63 kDa band corresponding to Transcription factor RelB was observed across across the cell lines tested. Whole cell extracts (30 µg lysate) of LNCaP (Lane 1), Raji (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:10,000 dilution using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RelB Polyclonal Antibody (Product # PA5-27679). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. RelB Polyclonal Antibody (Product # PA5-27679) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RelB Polyclonal Antibody (Product # PA5-27679). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB Polyclonal Antibody (Product # PA5-27679) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RelB was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RelB Polyclonal Antibody (Product # PA5-27679) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of RelB was performed in paraffin-embedded human breast carcinoma tissue using RelB Polyclonal Antibody (Product # PA5-27679) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RelB Polyclonal Antibody detects RelB protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. RelB stained by RelB Polyclonal Antibody (Product # PA5-27679) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cross-linked ChIP was performed with THP-1 chromatin extract treated with LPS (1.0 µg/mL for 3 h) and 5 µg of either normal rabbit IgG or RelB Polyclonal Antibody (Product # PA5-27679). The precipitated DNA was detected by PCR with primer set targeting to IL-1betapromoter.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RelB Polyclonal Antibody immunoprecipitates RelB protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. Control with 2 µg of preimmune rabbit IgG B. Immunoprecipitation of RelB protein by 2 µg of RelB Polyclonal Antibody (Product # PA5-27679) 7.5% SDS-PAGE The immunoprecipitated RelB protein was detected by RelB Polyclonal Antibody (Product # PA5-27679) diluted at 1:1,000.
