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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- LS-B9079 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ IL1R1 Antibody (aa474-503) LS-B9079
- Antibody type
- Polyclonal
- Description
- Protein A purified
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of lysate from HepG2 cell line, using IL1R-pY496. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of anti-IL1R in HL60 cell line lysates (35 ug/lane). IL1R (arrow) was detected using the purified antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-IL1R1 antibody IHC staining of human lung, macrophages. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
