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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AB-269-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 RI Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-1 RI in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse IL-1 RI is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- IL-1 beta/IL-1F2 Inhibition of Cell Proliferation and Neutralization by Human IL-1 RI Antibody. Recombinant Human IL-1 beta/ IL-1F2 (Catalog # 201-LB) inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Activity elicited by Recombinant Human IL-1 beta/IL-1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RI Polyclonal Antibody (Catalog # AB-269-NA). The ND50 is typically 5-10 µg/mL.
