Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
- Other assay [2]
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Validation data
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- Product number
- PA5-46930 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1R1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Dermal fibroblasts are the key sensors of aseptic skin inflammation through interleukin 1 release by lesioned keratinocytes.
Cordier-Dirikoc S, Pedretti N, Garnier J, Clarhaut-Charreau S, Ryffel B, Morel F, Bernard FX, Hamon de Almeida V, Lecron JC, Jégou JF
Frontiers in immunology 2022;13:984045
Frontiers in immunology 2022;13:984045
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of recombinant Human IL-1β/IL-1F2 inhibits proliferation in the A375s2 human melanoma cell line in a dose-dependent manner (orange line). Growth inhibition elicited by Recombinant Human IL-1β/IL-1F2 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RI Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46930). The ND50 is typically 0.5-2.0 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL1R1 in HUVEC human umbilical vein endothelial cells. Samples were incubated in IL1R1 polyclonal antibody (Product # PA5-46930) or control antibody followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of human IL-1beta/IL-1F2 is shown by increase in CPM (measure of growth) with increasing concentrations of IL-1 RI polyclonal antibody (PA5-46930).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL1R1 in HT-29 human colon adenocarcinoma cell line. Samples were incubated in IL1R1 polyclonal antibody (Product # PA5-46930). Recombinant Human IL‚1β/IL‚1F2 induces CXCL1/GROα secretion in the HT-29 human colon adenocarcinoma cell line in a dose-dependent manner(orange line), as measured by the Human CXCL1/GROα DuoSet. CXCL1/GROα secretion elicited by Recombinant Human IL‚1β/IL‚1F2 is neutralized (green line) by increasing concentrations of Human IL‚1 RI Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically < 5 μg/mL in the presence of 400 pg/mL Recombinant Human IL-1β/IL-1F2.