15421-1-AP

antibody from Proteintech Group

Targeting: ACLY ACL, ATPCL, CLATP

Provider product page for 15421-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [65]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 15421-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15421-1-AP, RRID:AB_2223741
Product name: ACLY antibody
Antibody type: Polyclonal
Description: KD/KO validated ACLY antibody (Cat. #15421-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IP, WB,ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

ACLY protein structure - 15421-1-AP shown in red.

Submitted references Lysine β-hydroxybutyrylation promotes lipid accumulation in alcoholic liver disease.

Chen N, Luo J, Zhou T, Shou Y, Du C, Song G, Xu L, Zhao K, Jin Y, Li C, Yu D
Biochemical pharmacology 2024 Oct;228:115936

Disease-associated astrocyte epigenetic memory promotes CNS pathology.

Lee HG, Rone JM, Li Z, Akl CF, Shin SW, Lee JH, Flausino LE, Pernin F, Chao CC, Kleemann KL, Srun L, Illouz T, Giovannoni F, Charabati M, Sanmarco LM, Kenison JE, Piester G, Zandee SEJ, Antel JP, Rothhammer V, Wheeler MA, Prat A, Clark IC, Quintana FJ
Nature 2024 Mar;627(8005):865-872

SIRT2-mediated deacetylation of ACLY promotes the progression of oesophageal squamous cell carcinoma.

Zhang X, Xu Y, Li S, Qin Y, Zhu G, Zhang Q, Zhang Y, Guan F, Fan T, Liu H
Journal of cellular and molecular medicine 2024 Mar;28(6):e18129

Pennogenin 3-O-β-Chacotrioside Attenuates Hypertrophied Lipid Accumulation by Enhancing Mitochondrial Oxidative Capacity.

Yu S, Lee HM, Lee J, Hwang JT, Choi HK, Lee YG
International journal of molecular sciences 2024 Mar 4;25(5)

CILP2 promotes hypertrophic scar through Snail acetylation by interaction with ACLY.

Wang J, Du J, Wang Y, Song Y, Wu J, Wang T, Yu Z, Song B
Biochimica et biophysica acta. Molecular basis of disease 2024 Jun;1870(5):167202

Therapeutic targeting of ACLY in T-ALL in vivo.

da Silva-Diz V, Singh A, Aleksandrova M, Kim O, Thai C, Lancho O, Nunes PR, Affronti H, Su X, Wellen KE, Herranz D
bioRxiv : the preprint server for biology 2024 Jun 20;

Disease-associated astrocyte epigenetic memory promotes CNS pathology.

Lee HG, Rone JM, Li Z, Akl CF, Shin SW, Lee JH, Flausino LE, Pernin F, Chao CC, Kleemann KL, Srun L, Illouz T, Giovannoni F, Charabati M, Sanmarco LM, Kenison JE, Piester G, Zandee SEJ, Antel J, Rothhammer V, Wheeler MA, Prat A, Clark IC, Quintana FJ
bioRxiv : the preprint server for biology 2024 Jan 5;

Prepared Radix Polygoni Multiflori and emodin alleviate lipid droplet accumulation in nonalcoholic fatty liver disease through MAPK signaling pathway inhibition.

Huang C, Zhang Y, Xu Y, Wei S, Yang T, Wang S, Li C, Lin H, Li X, Zhao S, Zhu L, Pan W
Aging 2024 Jan 26;16(3):2362-2384

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis.

Yang X, Gu C, Cai J, Li F, He X, Luo L, Xiao W, Hu B, Hu J, Qian H, Ren S, Zhang L, Zhu X, Yang L, Yang J, Yang Z, Zheng Y, Huang X, Wang Z
Redox biology 2024 Feb;69:103002

SHMT2 reduces fatty liver but is necessary for liver inflammation and fibrosis in mice.

Chen G, Zhou G, Zhai L, Bao X, Tiwari N, Li J, Mottillo E, Wang J
Communications biology 2024 Feb 12;7(1):173

The facilitating effects of KRT80 on chemoresistance, lipogenesis, and invasion of esophageal cancer.

Yun WJ, Li J, Yin NC, Zhang CY, Cui ZG, Zhang L, Zheng HC
Cancer biology & therapy 2024 Dec 31;25(1):2302162

Patulin Ameliorates Hypertrophied Lipid Accumulation and Lipopolysaccharide-Induced Inflammatory Response by Modulating Mitochondrial Respiration.

Hong S, Park SK, Lee J, Park SH, Kim YS, Park JH, Yu S, Lee YG
Antioxidants (Basel, Switzerland) 2023 Sep 11;12(9)

ACLY promotes gastric tumorigenesis and accelerates peritoneal metastasis of gastric cancer regulated by HIF-1A.

Yan Z, Liu K, Xu P, Chen Z, Zhang P, Pei S, Cheng Q, Huang S, Li B, Lv J, Xu Z, Xu H, Yang L, Zhang D
Cell cycle (Georgetown, Tex.) 2023 Oct;22(20):2288-2301

Hepatic conversion of acetyl-CoA to acetate plays crucial roles in energy stress.

Wang J, Wen Y, Zhao W, Zhang Y, Lin F, Ouyang C, Wang H, Yao L, Ma H, Zhuo Y, Huang H, Shi X, Feng L, Lin D, Jiang B, Li Q
eLife 2023 Oct 30;12

WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation.

Song K, Sun H, Tu B, Zhou Y, Lin LC, Liu ZY, Li R, Yang JJ, Zhang Y, Zhao JY, Tao H
iScience 2023 Oct 20;26(10):107931

High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate.

Zhou P, Chang WY, Gong DA, Xia J, Chen W, Huang LY, Liu R, Liu Y, Chen C, Wang K, Tang N, Huang AL
Cell metabolism 2023 Nov 7;35(11):1961-1975.e6

A bacteria-derived tetramerized protein ameliorates nonalcoholic steatohepatitis in mice via binding and relocating acetyl-coA carboxylase.

Lin Y, Yang M, Huang L, Yang F, Fan J, Qiang Y, Chang Y, Zhou W, Yan L, Xiong J, Ping J, Chen S, Men D, Li F
Cell reports 2023 Nov 28;42(11):113453

Acetyl-CoA is a key molecule for nephron progenitor cell pool maintenance.

Diniz F, Ngo NYN, Colon-Leyva M, Edgington-Giordano F, Hilliard S, Zwezdaryk K, Liu J, El-Dahr SS, Tortelote GG
Nature communications 2023 Nov 25;14(1):7733

Active post-transcriptional regulation and ACLY-mediated acetyl-CoA synthesis as a pivotal target of Shuang-Huang-Sheng-Bai formula for lung adenocarcinoma treatment.

Liu D, Dong C, Wang F, Liu W, Jin X, Qi SL, Liu L, Jin Q, Wang S, Wu J, Wang C, Yang J, Deng H, Cai Y, Yang L, Qin J, Zhang C, Yang X, Wang MS, Yu G, Xue YW, Wang Z, Ge GB, Xu Z, Chen WL
Phytomedicine : international journal of phytotherapy and phytopharmacology 2023 May;113:154732

Acetyl-CoA carboxylase 1 is a suppressor of the adipocyte thermogenic program.

Guilherme A, Rowland LA, Wetoska N, Tsagkaraki E, Santos KB, Bedard AH, Henriques F, Kelly M, Munroe S, Pedersen DJ, Ilkayeva OR, Koves TR, Tauer L, Pan M, Han X, Kim JK, Newgard CB, Muoio DM, Czech MP
Cell reports 2023 May 30;42(5):112488

Acetylcarnitine shuttling links mitochondrial metabolism to histone acetylation and lipogenesis.

Izzo LT, Trefely S, Demetriadou C, Drummond JM, Mizukami T, Kuprasertkul N, Farria AT, Nguyen PTT, Murali N, Reich L, Kantner DS, Shaffer J, Affronti H, Carrer A, Andrews A, Capell BC, Snyder NW, Wellen KE
Science advances 2023 May 3;9(18):eadf0115

TRIM21 attenuates renal carcinoma lipogenesis and malignancy by regulating SREBF1 protein stability.

Chen X, Yong H, Chen M, Deng C, Wang P, Chu S, Li M, Hou P, Zheng J, Li Z, Bai J
Journal of experimental & clinical cancer research : CR 2023 Jan 25;42(1):34

LncHLEF promotes hepatic lipid synthesis through miR-2188-3p/GATA6 axis and encoding peptides and enhances intramuscular fat deposition via exosome.

Guo Y, Tian W, Wang D, Yang L, Wang Z, Wu X, Zhi Y, Zhang K, Wang Y, Li Z, Jiang R, Sun G, Li G, Tian Y, Wang H, Kang X, Liu X, Li H
International journal of biological macromolecules 2023 Dec 31;253(Pt 5):127061

Deletion of ACLY Disrupts Histone Acetylation and IL-10 Secretion in Trophoblasts, Which Inhibits M2 Polarization of Macrophages: A Possible Role in Recurrent Spontaneous Abortion.

Chen X, Song QL, Li ZH, Ji R, Wang JY, Ge C, Xiao ZN, Guo DY, Yang J
Oxidative medicine and cellular longevity 2022;2022:5216786

Impact of ATP-citrate lyase catalytic activity and serine 455 phosphorylation on histone acetylation and inflammatory responses in human monocytic THP-1 cells.

Dominguez M, Truemper V, Mota AC, Brüne B, Namgaladze D
Frontiers in immunology 2022;13:906127

PRMT5 confers lipid metabolism reprogramming, tumour growth and metastasis depending on the SIRT7-mediated desuccinylation of PRMT5 K387 in tumours.

Yuan HF, Zhao M, Zhao LN, Yun HL, Yang G, Geng Y, Wang YF, Zheng W, Yuan Y, Song TQ, Niu JQ, Zhang XD
Acta pharmacologica Sinica 2022 Sep;43(9):2373-2385

PTPRO represses colorectal cancer tumorigenesis and progression by reprogramming fatty acid metabolism.

Dai W, Xiang W, Han L, Yuan Z, Wang R, Ma Y, Yang Y, Cai S, Xu Y, Mo S, Li Q, Cai G
Cancer communications (London, England) 2022 Sep;42(9):848-867

circPRKAA1 activates a Ku80/Ku70/SREBP-1 axis driving de novo fatty acid synthesis in cancer cells.

Li Q, Yao H, Wang Y, Wu Y, Thorne RF, Zhu Y, Wu M, Liu L
Cell reports 2022 Nov 22;41(8):111707

The enhancer RNA ADCY10P1 is associated with the progression of ovarian cancer.

Mo J, Zhang L, Li H, Duan H, Wang D, Zhao X, Xie Y
Journal of ovarian research 2022 May 14;15(1):61

The CBL-LSD1-CXCL8 axis regulates methionine metabolism in glioma.

Chang J, Wang L, Zhou X, Yuan J, Xu W
Cytokine 2022 Mar;151:155789

Albumin knockout mice exhibit reduced plasma free fatty acid concentration and enhanced insulin sensitivity.

Abdollahi A, Dowden BN, Buhman KK, Zembroski AS, Henderson GC
Physiological reports 2022 Mar;10(5):e15161

Novel phosphanegold(I) thiolate complexes suppress de novo lipid synthesis in human lung cancer.

Luo M, Ma X, Jiang W, Zhang J, Liu W, Wei S, Liu H
European journal of medicinal chemistry 2022 Mar 15;232:114168

Endurance exercise-mediated metabolic reshuffle attenuates high-caloric diet-induced non-alcoholic fatty liver disease.

Cook JJ, Wei M, Segovia B, Cosio-Lima L, Simpson J, Taylor S, Koh Y, Kim S, Lee Y
Annals of hepatology 2022 Jul-Aug;27(4):100709

Quantitative subcellular acyl-CoA analysis reveals distinct nuclear metabolism and isoleucine-dependent histone propionylation.

Trefely S, Huber K, Liu J, Noji M, Stransky S, Singh J, Doan MT, Lovell CD, von Krusenstiern E, Jiang H, Bostwick A, Pepper HL, Izzo L, Zhao S, Xu JP, Bedi KC Jr, Rame JE, Bogner-Strauss JG, Mesaros C, Sidoli S, Wellen KE, Snyder NW
Molecular cell 2022 Jan 20;82(2):447-462.e6

Nanog mediated by FAO/ACLY signaling induces cellular dormancy in colorectal cancer cells.

Zhang M, Peng R, Wang H, Yang Z, Zhang H, Zhang Y, Wang M, Wang H, Lin J, Zhao Q, Liu J
Cell death & disease 2022 Feb 17;13(2):159

Glucose-6-phosphate dehydrogenase exerts antistress effects independently of its enzymatic activity.

Jin X, Li X, Li L, Zhong B, Hong Y, Niu J, Li B
The Journal of biological chemistry 2022 Dec;298(12):102587

Di-2-ethylhexyl phthalate-induced miR-155-5p promoted lipid metabolism via inhibiting cAMP/PKA signaling pathway in human trophoblastic HTR-8/Svneo cells.

Gu X, Liu H, Luo W, Wang X, Wang H, Li L
Reproductive toxicology (Elmsford, N.Y.) 2022 Dec;114:22-31

Bempedoic acid, an ATP citrate lyase inhibitor, reduces intimal hyperplasia via activation of AMPKα signaling pathway.

Liu W, Liu M, Xiong H, Xia L, Yang Q, Chen M, Cai Y, Li S
International immunopharmacology 2022 Dec;113(Pt A):109392

Hypoxia promotes osteogenesis by facilitating acetyl-CoA-mediated mitochondrial-nuclear communication.

Pouikli A, Maleszewska M, Parekh S, Yang M, Nikopoulou C, Bonfiglio JJ, Mylonas C, Sandoval T, Schumacher AL, Hinze Y, Matic I, Frezza C, Tessarz P
The EMBO journal 2022 Dec 1;41(23):e111239

Mitofusin 2 Deficiency Causes Pro-Inflammatory Effects in Human Primary Macrophages.

Khodzhaeva V, Schreiber Y, Geisslinger G, Brandes RP, Brüne B, Namgaladze D
Frontiers in immunology 2021;12:723683

Novel SREBP1 inhibitor cinobufotalin suppresses proliferation of hepatocellular carcinoma by targeting lipogenesis.

Meng H, Shen M, Li J, Zhang R, Li X, Zhao L, Huang G, Liu J
European journal of pharmacology 2021 Sep 5;906:174280

Metformin alleviates inflammation through suppressing FASN-dependent palmitoylation of Akt.

Xiong W, Sun KY, Zhu Y, Zhang X, Zhou YH, Zou X
Cell death & disease 2021 Oct 12;12(10):934

ER resident protein 44 promotes malignant phenotype in nasopharyngeal carcinoma through the interaction with ATP citrate lyase.

Tian H, Shi S, You B, Zhang Q, Gu M, You Y
Journal of translational medicine 2021 Feb 16;19(1):77

Nuclear-translocation of ACLY induced by obesity-related factors enhances pyrimidine metabolism through regulating histone acetylation in endometrial cancer.

Dai M, Yang B, Chen J, Liu F, Zhou Y, Zhou Y, Xu Q, Jiang S, Zhao S, Li X, Zhou X, Yang Q, Li J, Wang Y, Zhang Z, Teng Y
Cancer letters 2021 Aug 10;513:36-49

Control of Adipocyte Thermogenesis and Lipogenesis through β3-Adrenergic and Thyroid Hormone Signal Integration.

Guilherme A, Yenilmez B, Bedard AH, Henriques F, Liu D, Lee A, Goldstein L, Kelly M, Nicoloro SM, Chen M, Weinstein L, Collins S, Czech MP
Cell reports 2020 May 5;31(5):107598

Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate.

Zhao S, Jang C, Liu J, Uehara K, Gilbert M, Izzo L, Zeng X, Trefely S, Fernandez S, Carrer A, Miller KD, Schug ZT, Snyder NW, Gade TP, Titchenell PM, Rabinowitz JD, Wellen KE
Nature 2020 Mar;579(7800):586-591

Acetyl-CoA Derived from Hepatic Peroxisomal β-Oxidation Inhibits Autophagy and Promotes Steatosis via mTORC1 Activation.

He A, Chen X, Tan M, Chen Y, Lu D, Zhang X, Dean JM, Razani B, Lodhi IJ
Molecular cell 2020 Jul 2;79(1):30-42.e4

Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells.

Yang N, Li C, Li H, Liu M, Cai X, Cao F, Feng Y, Li M, Wang X
Frontiers in pharmacology 2019;10:709

ACLY facilitates colon cancer cell metastasis by CTNNB1.

Wen J, Min X, Shen M, Hua Q, Han Y, Zhao L, Liu L, Huang G, Liu J, Zhao X
Journal of experimental & clinical cancer research : CR 2019 Sep 12;38(1):401

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Zhang Y, Li C, Hu C, Wu Q, Cai Y, Xing S, Lu H, Wang L, Huang D, Sun L, Li T, He X, Zhong X, Wang J, Gao P, Smith ZJ, Jia W, Zhang H
EMBO reports 2019 Oct 4;20(10):e48115

Adipocyte ACLY Facilitates Dietary Carbohydrate Handling to Maintain Metabolic Homeostasis in Females.

Fernandez S, Viola JM, Torres A, Wallace M, Trefely S, Zhao S, Affronti HC, Gengatharan JM, Guertin DA, Snyder NW, Metallo CM, Wellen KE
Cell reports 2019 May 28;27(9):2772-2784.e6

Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism.

Trefely S, Liu J, Huber K, Doan MT, Jiang H, Singh J, von Krusenstiern E, Bostwick A, Xu P, Bogner-Strauss JG, Wellen KE, Snyder NW
Molecular metabolism 2019 Dec;30:61-71

Polarization of Human Macrophages by Interleukin-4 Does Not Require ATP-Citrate Lyase.

Namgaladze D, Zukunft S, Schnütgen F, Kurrle N, Fleming I, Fuhrmann D, Brüne B
Frontiers in immunology 2018;9:2858

DJ-1 Deficiency Protects Hepatic Steatosis by Enhancing Fatty Acid Oxidation in Mice.

Xu M, Wu H, Li M, Wen Y, Yu C, Xia L, Xia Q, Kong X
International journal of biological sciences 2018;14(13):1892-1900

Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals.

Liu X, Cooper DE, Cluntun AA, Warmoes MO, Zhao S, Reid MA, Liu J, Lund PJ, Lopes M, Garcia BA, Wellen KE, Kirsch DG, Locasale JW
Cell 2018 Oct 4;175(2):502-513.e13

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Wang L, Zhang T, Wang L, Cai Y, Zhong X, He X, Hu L, Tian S, Wu M, Hui L, Zhang H, Gao P
The EMBO journal 2017 May 15;36(10):1330-1347

Acetyl-CoA synthetase regulates histone acetylation and hippocampal memory.

Mews P, Donahue G, Drake AM, Luczak V, Abel T, Berger SL
Nature 2017 Jun 15;546(7658):381-386

Nuclear Acetyl-CoA Production by ACLY Promotes Homologous Recombination.

Sivanand S, Rhoades S, Jiang Q, Lee JV, Benci J, Zhang J, Yuan S, Viney I, Zhao S, Carrer A, Bennett MJ, Minn AJ, Weljie AM, Greenberg RA, Wellen KE
Molecular cell 2017 Jul 20;67(2):252-265.e6

ACSS2-mediated acetyl-CoA synthesis from acetate is necessary for human cytomegalovirus infection.

Vysochan A, Sengupta A, Weljie AM, Alwine JC, Yu Y
Proceedings of the National Academy of Sciences of the United States of America 2017 Feb 21;114(8):E1528-E1535

The microRNA-182-PDK4 axis regulates lung tumorigenesis by modulating pyruvate dehydrogenase and lipogenesis.

Li G, Li M, Hu J, Lei R, Xiong H, Ji H, Yin H, Wei Q, Hu G
Oncogene 2017 Feb 16;36(7):989-998

CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.

Gu Z, Zhang H, Li Y, Shen S, Yin X, Zhang W, Cheng R, Zhang Y, Zhang X, Chen H, Huang B, Cao Y
Oncotarget 2017 Dec 26;8(69):114328-114343

Acetyl-coenzyme A carboxylase alpha promotion of glucose-mediated fatty acid synthesis enhances survival of hepatocellular carcinoma in mice and patients.

Wang MD, Wu H, Fu GB, Zhang HL, Zhou X, Tang L, Dong LW, Qin CJ, Huang S, Zhao LH, Zeng M, Wu MC, Yan HX, Wang HY
Hepatology (Baltimore, Md.) 2016 Apr;63(4):1272-86

Knockdown of PKM2 Suppresses Tumor Growth and Invasion in Lung Adenocarcinoma.

Sun H, Zhu A, Zhang L, Zhang J, Zhong Z, Wang F
International journal of molecular sciences 2015 Oct 15;16(10):24574-87

Mutations in CIC and IDH1 cooperatively regulate 2-hydroxyglutarate levels and cell clonogenicity.

Chittaranjan S, Chan S, Yang C, Yang KC, Chen V, Moradian A, Firme M, Song J, Go NE, Blough MD, Chan JA, Cairncross JG, Gorski SM, Morin GB, Yip S, Marra MA
Oncotarget 2014 Sep 15;5(17):7960-79

Human cytomegalovirus infection induces adipocyte-like lipogenesis through activation of sterol regulatory element binding protein 1.

Yu Y, Maguire TG, Alwine JC
Journal of virology 2012 Mar;86(6):2942-9

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 15421-1-AP(ACLY antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using ACLY antibody 15421-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line