PAB21474

antibody from Abnova Corporation

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21474

Provider

Abnova Corporation

Proper citation

Product name

ACLY polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ACLY.

Antigen sequence

YICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSG
ASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLG
GVNELANYGEYSGAPSEQQTYDYAKTILSLM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with ACLY polyclonal antibody (Cat # PAB21474) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with ACLY polyclonal antibody (Cat # PAB21474) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli, plasma membrane and cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with ACLY polyclonal antibody (Cat # PAB21474) shows strong cytoplasmic and nuclear positivity in islet cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)