PA5-29495

antibody from Invitrogen Antibodies

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29495

Provider

Invitrogen Antibodies

Product name

ATP Citrate Lyase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, Raji, mouse brain, PC-12, Rat-2. Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (91%), Pig (98%), Chicken (95%), Sheep (99%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

BCAA Supplementation in Mice with Diet-induced Obesity Alters the Metabolome Without Impairing Glucose Homeostasis.

Lee J, Vijayakumar A, White PJ, Xu Y, Ilkayeva O, Lynch CJ, Newgard CB, Kahn BB
Endocrinology 2021 Jul 1;162(7)

---

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Hughey CC, James FD, Wang Z, Goelzer M, Wasserman DH
Molecular metabolism 2019 May;23:1-13

---

Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates.

Hughey CC, Trefts E, Bracy DP, James FD, Donahue EP, Wasserman DH
The Journal of biological chemistry 2018 Jul 27;293(30):11944-11954

---

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Plubell DL, Wilmarth PA, Zhao Y, Fenton AM, Minnier J, Reddy AP, Klimek J, Yang X, David LL, Pamir N
Molecular & cellular proteomics : MCP 2017 May;16(5):873-890

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ATP Citrate Lyase was performed in HeLa cells fixed in ice cold MeOH for 5 min. Green: ATP Citrate Lyase Polyclonal Antibody (Product # PA5 29495) diluted at 1:200. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ATP Citrate Lyase was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29495) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL