RQ5221

antibody from NSJ Bioreagents

Targeting: ACLY ACL, ATPCL, CLATP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5221

Provider

NSJ Bioreagents

Product name

ATP Citrate Lyase Antibody

Antibody type

Monoclonal

Description

This highly specific ATP Citrate Lyase antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry/Immunofluorescence applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

5I2

Vial size

100 ul

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the ATP Citrate Lyase antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta, 2) U-87 MG, 3) HEK293, 4) Caco-2, 5) HL-60, 6) Raji, 7) ThP-1 and 8) PANC-1 lysate with ATP Citrate Lyase antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat 1) lung, 2) testis, 3) kidney, 4) brain, and mouse 5) lung, 6) testis and 7) kidney lysate with ATP Citrate Lyase antibody.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human MCF7 cells with ATP Citrate Lyase antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human pancreas with ATP Citrate Lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human testis cancer with ATP Citrate Lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse pancreas with ATP Citrate Lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE rat pancreas with ATP Citrate Lyase antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A549 cells with ATP Citrate Lyase antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ATP Citrate Lyase antibody.