LS-B374

antibody from LSBio

Targeting: GYS1 GSY, GYS

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-B374

Provider

LSBio

Proper citation

LifeSpan Cat#LS-B374, RRID:AB_2232894

Product name

IHC-plus™ GYS1 / Glycogen Synthase Antibody (phospho-Ser640) LS-B374

Antibody type

Polyclonal

Description

Affinity purified

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 4°C or -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-Muscle Glycogen Synthase pS640 Antibody - Western Blot. Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase (GS) at pS640 was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 um (lanes 2, 3 and 4 respectively) for 15 or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45. After washing, a 1:5000 dilution of HRP conjugated Gt-a-Rabbit IgG (LS-C60865) preceded color development using Amershams substrate system. Other detection methods will yield similar results.