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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA1-26469 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-GYS1 (Ser640) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-26469 detects Glycogen Synthase (phospho S640) from human and mouse samples.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 Phospho-GYS1 (Ser640) Polyclonal Antibody (Product # PA1-26469) was used at a 1:1,000 dilution to detect human muscle GS by Western blot. Approximately 12 µL of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 µM (lanes 2, 3 and 4 respectively) for 15 or CLA treated at 4 nm, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (Product # PA1-28773) preceded color development using Amershams substrate system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of Phospho-GYS1 was performed in formalin fixed, paraffin embedded human prostate tissue. Antigen retrieval was heat-induced, and a Phospho-GYS1 (Ser640) Polyclonal Antibody (Product # PA1-26469) was used at 5 µg/mL. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
