Antibody data
- Antibody Data
- Antigen structure
- References [33]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- 10566-1-AP - Provider product page

- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10566-1-AP, RRID:AB_2116401
- Product name
- GYS1 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated GYS1 antibody (Cat. #10566-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references 4-Methylesculetin Ameliorates Hepatic Insulin Resistance in HepG2 Cells Through AMPK/FOXO1, PI3K/AKT/GSK3β Pathways and SIRT1/NOX4 Axis.
A Metabolism-Driven Prognostic Model and PSMD14-SP1-GYS1 Axis Reveal Therapeutic Vulnerabilities in Melanoma.
Based on disulfide accumulation, leveraging a THBS1-signature to improve the outcome of head and neck carcinoma patients.
Molecular subtypes and prognostic signature rooted in disulfidptosis highlight tumor microenvironment in lung adenocarcinoma.
Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA.
Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients.
Xiasangju alleviates hepatic insulin resistance in db/db mice via AMPK pathway: Mechanisms and active components study.
The inhibition of placental mTOR signaling leads to fetal growth restriction with abnormal glucose metabolism in different anatomical regions of placentas.
Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8(+) memory T cells.
α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice.
Blocking glycogen synthase 1 in white adipose tissue alleviates hypermetabolism following severe burn injury through inhibition of JAK2 by UDPG.
Multi-layered transcriptional control of glycogen metabolism coordinates thermogenic remodeling of white adipocytes in male mice.
Neuromodulatory control of energy reserves in dopaminergic neurons.
Neuromodulator control of energy reserves in dopaminergic neurons.
Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis.
Radix Astragali decoction improves liver regeneration by upregulating hepatic expression of aquaporin-9.
Identification and validation of a disulfidptosis-related genes prognostic signature in lung adenocarcinoma.
Astragaloside IV and cycloastragenol promote liver regeneration through regulation of hepatic oxidative homeostasis and glucose/lipid metabolism.
Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy.
PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.
Construction and experimental validation of a signature for predicting prognosis and immune infiltration analysis of glioma based on disulfidptosis-related lncRNAs.
Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction.
Douchi Peptides VY and SFLLR Improve Glucose Homeostasis and Gut Dysbacteriosis in High-Fat Diet-Induced Insulin Resistant Mice.
Identification of novel immune subtypes and potential hub genes of patients with psoriasis.
Prenatal methamphetamine exposure causes dysfunction in glucose metabolism and low birthweight.
Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat.
Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.
Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.
Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.
A single centrifugation method for isolating fat droplets from cells and tissues.
Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.
Su X, Du Y, Yang Y, Zhao Y, Zhao H, Xie J, Shan Z, Wang M, Huang Z, Fu W, Wan A, An Y, Zhao B
Journal of diabetes research 2026;2026(1):e8023146
Journal of diabetes research 2026;2026(1):e8023146
A Metabolism-Driven Prognostic Model and PSMD14-SP1-GYS1 Axis Reveal Therapeutic Vulnerabilities in Melanoma.
Xie J, Zhao S, Wu D, Ma C, Yan W, Zhang P, Lu Q, Wan Z, Tang Q, Li L, Wang M, He Y
The Journal of investigative dermatology 2026 Apr;146(4):1074-1088.e5
The Journal of investigative dermatology 2026 Apr;146(4):1074-1088.e5
Based on disulfide accumulation, leveraging a THBS1-signature to improve the outcome of head and neck carcinoma patients.
Jin Y, Wang Z, Zhou C, Wen M, Zhou Y, Huang S
International journal of biological macromolecules 2025 Sep 15;328(Pt 2):147679
International journal of biological macromolecules 2025 Sep 15;328(Pt 2):147679
Molecular subtypes and prognostic signature rooted in disulfidptosis highlight tumor microenvironment in lung adenocarcinoma.
Xia W, Liu J, Chen R, Feng J, Wu L, Wang Y, Wang X, Song C, Mao W
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2025 Oct 30;37(5):796-820
Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 2025 Oct 30;37(5):796-820
Screening of key genes related to disulfidptosis in psoriasis based on the analysis of WGCNA.
Li Q, Xu P, Piao Y, Wang Z, Lv C
Scientific reports 2025 Nov 28;15(1):45579
Scientific reports 2025 Nov 28;15(1):45579
Patient-derived organotypic tissue cultures as a platform to evaluate metabolic reprogramming in breast cancer patients.
Fan TW, Yan J, Goncalves CFL, Islam JMM, Lin P, Kaddah MMY, Higashi RM, Lane AN, Wang X, Zhu C
The Journal of biological chemistry 2025 May;301(5):108495
The Journal of biological chemistry 2025 May;301(5):108495
Xiasangju alleviates hepatic insulin resistance in db/db mice via AMPK pathway: Mechanisms and active components study.
An Y, Zhao H, He C, Shi L, Su X, Zhang H, Huang Y, Shan Z, Wang M, Du Y, Xie J, Zhao Y, Yang Y, Huang Z, Wan A, Zhao Y, Zhao B
International immunopharmacology 2025 May 27;156:114675
International immunopharmacology 2025 May 27;156:114675
The inhibition of placental mTOR signaling leads to fetal growth restriction with abnormal glucose metabolism in different anatomical regions of placentas.
Xu Q, Wang J, Li Y, Lei H, Jin N, Lu J, Qian C, Zhang J, Dong J, Wang X
Placenta 2025 May 2;164:31-40
Placenta 2025 May 2;164:31-40
Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8(+) memory T cells.
Zhou Y, Zhang C, He L, Kang Y, Wang D, Wang S, Ling Z, Chen J, Zhou N, Zhou L, Wang Z, Zhang C, Tang K, Zhang H, Ma J, Qin B, Cui S, Lv J, Huang B
Molecular cell 2025 Jul 3;85(13):2535-2549.e10
Molecular cell 2025 Jul 3;85(13):2535-2549.e10
α-Klotho Supplementation Mitigates Cumulative Exercise-Induced Fatigue via Coordinated NRF2-Mediated Antioxidant Defense and AKT/GS-Driven Hepatic Glycogen Supercompensation in Mice.
Zheng L, Wang Y, Xiao Z, Rao Z, Shi R
International journal of molecular sciences 2025 Dec 30;27(1)
International journal of molecular sciences 2025 Dec 30;27(1)
Blocking glycogen synthase 1 in white adipose tissue alleviates hypermetabolism following severe burn injury through inhibition of JAK2 by UDPG.
Zhuo S, Wang Z, Yang L, Yuan Y, Hu D, Chen Y
Cell reports 2025 Dec 23;44(12):116577
Cell reports 2025 Dec 23;44(12):116577
Multi-layered transcriptional control of glycogen metabolism coordinates thermogenic remodeling of white adipocytes in male mice.
Fu H, Lee S, Zemke NR, Fan W, Wang Y, Garza J, Tin D, Villao B, Zhang B, Ma X, Zhang J, Dong T, Ren Y, Downes M, Evans RM, Ren B, Saltiel AR
Nature communications 2025 Dec 16;17(1):809
Nature communications 2025 Dec 16;17(1):809
Neuromodulatory control of energy reserves in dopaminergic neurons.
Pulido C, Gentry MS, Ryan TA
Proceedings of the National Academy of Sciences of the United States of America 2025 Dec 16;122(50):e2523019122
Proceedings of the National Academy of Sciences of the United States of America 2025 Dec 16;122(50):e2523019122
Neuromodulator control of energy reserves in dopaminergic neurons.
Pulido C, Gentry MS, Ryan TA
bioRxiv : the preprint server for biology 2025 Aug 19;
bioRxiv : the preprint server for biology 2025 Aug 19;
Si-Ni-San promotes liver regeneration by maintaining hepatic oxidative equilibrium and glucose/lipid metabolism homeostasis.
Yang X, Zhang J, Li Y, Hu H, Li X, Ma T, Zhang B
Journal of ethnopharmacology 2024 May 23;326:117918
Journal of ethnopharmacology 2024 May 23;326:117918
Radix Astragali decoction improves liver regeneration by upregulating hepatic expression of aquaporin-9.
Li Y, Yang X, Bao T, Sun X, Li X, Zhu H, Zhang B, Ma T
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jan;122:155166
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Jan;122:155166
Identification and validation of a disulfidptosis-related genes prognostic signature in lung adenocarcinoma.
Zhang Y, Sun J, Li M, Hou L, Wang Z, Dong H, Xu W, Jiang R, Geng Y, Guan C, Zhu Z, Wang H, Gong Q, Zhang G
Heliyon 2024 Jan 15;10(1):e23502
Heliyon 2024 Jan 15;10(1):e23502
Astragaloside IV and cycloastragenol promote liver regeneration through regulation of hepatic oxidative homeostasis and glucose/lipid metabolism.
Li Y, Yang X, Li X, Wang S, Chen P, Ma T, Zhang B
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Dec;135:156165
Phytomedicine : international journal of phytotherapy and phytopharmacology 2024 Dec;135:156165
Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy.
Fan H, Tan X, Xu S, Zeng Y, Zhang H, Shao T, Zhao R, Zhou P, Bo X, Fan J, Fu Y, Ding X, Zhou Y
Molecular medicine (Cambridge, Mass.) 2024 Dec 19;30(1):249
Molecular medicine (Cambridge, Mass.) 2024 Dec 19;30(1):249
PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer.
Ji Q, Li H, Cai Z, Yuan X, Pu X, Huang Y, Fu S, Chu L, Jiang C, Xue J, Zhang X, Li R
International journal of biological sciences 2023;19(6):1894-1909
International journal of biological sciences 2023;19(6):1894-1909
Construction and experimental validation of a signature for predicting prognosis and immune infiltration analysis of glioma based on disulfidptosis-related lncRNAs.
Guo Y, Jiang Z, Chen Q, Xie D, Zhou Y, Yin W, Wang Z, Wang B, Ren C, Jiang X
Frontiers in immunology 2023;14:1291385
Frontiers in immunology 2023;14:1291385
Depressed Proximal Glycolysis in Myocardium Of Human Heart Failure with Preserved Ejection Fraction.
Koleini N, Meddeb M, Keykhaei M, Kwon S, Zhao L, Hahn V, Sharma K, Pearce EL, Kass DA
medRxiv : the preprint server for health sciences 2023 Oct 2;
medRxiv : the preprint server for health sciences 2023 Oct 2;
Douchi Peptides VY and SFLLR Improve Glucose Homeostasis and Gut Dysbacteriosis in High-Fat Diet-Induced Insulin Resistant Mice.
Yu S, Wang W, Wang H, Bu T, Liu L, Zhao R, Li S, Liu D, Wu J
Molecular nutrition & food research 2023 May;67(10):e2200681
Molecular nutrition & food research 2023 May;67(10):e2200681
Identification of novel immune subtypes and potential hub genes of patients with psoriasis.
Li Y, Li L, Tian Y, Luo J, Huang J, Zhang L, Zhang J, Li X, Hu L
Journal of translational medicine 2023 Mar 8;21(1):182
Journal of translational medicine 2023 Mar 8;21(1):182
Prenatal methamphetamine exposure causes dysfunction in glucose metabolism and low birthweight.
Doi M, Nakama N, Sumi T, Usui N, Shimada S
Frontiers in endocrinology 2022;13:1023984
Frontiers in endocrinology 2022;13:1023984
Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat.
Liu Z, Zhang L, Qian C, Zhou Y, Yu Q, Yuan J, Lv Y, Zhang L, Chang X, Li Y, Liu Y
Metabolism: clinical and experimental 2022 Nov;136:155310
Metabolism: clinical and experimental 2022 Nov;136:155310
Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.
Fan TW, Daneshmandi S, Cassel TA, Uddin MB, Sledziona J, Thompson PT, Lin P, Higashi RM, Lane AN
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690
Journal of immunology (Baltimore, Md. : 1950) 2022 Nov 1;209(9):1674-1690
Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.
Gu Z, Zhang H, Guo X, Cao Y
The Journal of investigative dermatology 2020 Aug;140(8):1513-1523.e5
The Journal of investigative dermatology 2020 Aug;140(8):1513-1523.e5
Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.
Wang F, Chang C, Li R, Zhang Z, Jiang H, Zeng N, Li D, Chen L, Xiao Y, Chen W, Wang Q
Toxicology 2019 Aug 1;424:152236
Toxicology 2019 Aug 1;424:152236
ROS-mediated glucose metabolic reprogram induces insulin resistance in type 2 diabetes.
Dong K, Ni H, Wu M, Tang Z, Halim M, Shi D
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
Biochemical and biophysical research communications 2016 Aug 5;476(4):204-211
Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.
Zhu H, Sun A, Zou Y, Ge J
Arteriosclerosis, thrombosis, and vascular biology 2014 Apr;34(4):870-6
Arteriosclerosis, thrombosis, and vascular biology 2014 Apr;34(4):870-6
A single centrifugation method for isolating fat droplets from cells and tissues.
Harris LLS, Shew TM, Skinner JR, Wolins NE
Journal of lipid research 2012 May;53(5):1021-1025
Journal of lipid research 2012 May;53(5):1021-1025
Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.
Hall AM, Brunt EM, Chen Z, Viswakarma N, Reddy JK, Wolins NE, Finck BN
Journal of lipid research 2010 Mar;51(3):554-63
Journal of lipid research 2010 Mar;51(3):554-63
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 10566-1-AP(GYS1 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunofluorescent analysis of HepG2 cells, using GYS1 antibody 10566-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image

- Experimental details
- Immunohistochemical of paraffin-embedded human prostate cancer using 10566-1-AP(GYS1 antibody) at dilution of 1:50 (under 25x lens)
- Sample type
- tissue