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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- GTX22479 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX22479, RRID:AB_374324
- Product name
- Glycogen synthase 1 (phospho Ser640) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Phenotype consequences of myophosphorylase dysfunction: insights from the McArdle mouse model.
Brull A, de Luna N, Blanco-Grau A, Lucia A, Martin MA, Arenas J, Martí R, Andreu AL, Pinós T
The Journal of physiology 2015 Jun 15;593(12):2693-706
The Journal of physiology 2015 Jun 15;593(12):2693-706
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Affinity Purified Phospho-specific antibody to human muscle Glycogen Synthase at pS640 (GTX22479) was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 ?M (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG (GTX27090) preceded color development using Amersham's substrate system.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry with Anti-Glycogen Synthase at pS641antibody (GTX22479) Tissue: Human Prostate Fixation: formalin-fixed, paraffin-embedded tissue Antigen retrieval: heat-induced Primary antibody: 5 ?g/ml Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
