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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- BS4857 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- PAK2 (phospho S20) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human PAK2 around the phosphorylation site of Serine 20.
- Description
- Three isoforms of serine/threonine kinases, designated Ï«PAK p68, ÉÇPAK p65 and ÎÛPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The Ï«, ÉÇ and ÎÛPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on ÎÛPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with ÎÛPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of p-PAK2 (S20) pAb at 1:500 dilutionLane1:HEK293T cell lysate treated with EGF(0.1ng/ML,30mins)Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ML,30mins)Lane3:H9C2 cell lysate treated with EGF(0.1ng/ML,30mins)
