NBP1-76720

antibody from Novus Biologicals

Targeting: PAK2 PAK65, PAKgamma

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-76720

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-76720, RRID:AB_11012387

Product name

Rabbit Polyclonal PAK2 Antibody

Antibody type

Polyclonal

Description

Peptide affinity purified.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PAK2 Antibody [NBP1-76720] - Analysis of PAK2 in rat bladder tissue lysate with PAK2 antibody at (A) 0.5 and (B) 1 ug/mL.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PAK2 Antibody [NBP1-76720] - Western Blot Validation in Mouse Tissues. Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL per lane), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PAK2 Antibody [NBP1-76720] - Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL per lane), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: PAK2 Antibody [NBP1-76720] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: PAK2 NBP1-76720 (1 ug/mL), PAK2 NBP1-76721 (1 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: PAK2 Antibody [NBP1-76720] - Mouse spleen tissue with PAK2 antibody at 10 ug/ml.