PA5-20274

antibody from Invitrogen Antibodies

Targeting: PAK2 PAK65, PAKgamma

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-20274

Provider

Invitrogen Antibodies

Product name

PAK2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

A suggested positive control is Jurkat cell lysate.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Jurkat lysate using a PAK2 polyclonal antibody (Product # PA5-20274) at (A) 0.5 and (B) 1 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Human, Mouse and Rat Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PAK2 Polyclonal Antibody (Product # PA5-20274) (1 µg/mL per lane), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Mouse Tissues. Loading: 15 µg of lysates per lane. Antibodies: PAK2 Polyclonal Antibody (Product # PA5-20274) (1 µg/mL per lane), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot Validation in Rat Bladder Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: PAK2 Polyclonal Antibody (Product # PA5-20274) (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of mouse spleen cells using a PAK2 polyclonal antibody (Product # PA5-20274) at a 20 µg/mL dilution.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse spleen labeling PAK2 with PAK2 Polyclonal Antibody (Product # PA5-20274) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using PAK2 Polyclonal Antibody (Product # PA5-20274) at 10 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.