EB06695

antibody from Everest Biotech

Targeting: FCGR2B CD32, CD32B, FCG2, FCGR2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

EB06695

Provider

Everest Biotech

Proper citation

Everest Biotech Cat#EB06695, RRID:AB_2103601

Product name

Goat anti-CD32 / FCGR2B Antibody

Antibody type

Polyclonal

Description

Goat anti-CD32 / FCGR2B Antibody

Reactivity

Human

Host

Goat

Antigen sequence

C-PDALEEPDDQNRI


Epitope

C Terminus

Isotype

IgG

Vial size

100µg specific antibody in 200µl

Concentration

0.5 mg/ml

Storage

Aliquot and store at -20°C. Minimize freezing and thawing.

Western blot

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB06695 (0.3µg/ml) staining of K562 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

HEK293 overexpressing CD32 (RC211982) and probed with EB06695 (mock transfection in first lane), tested by Origene.

Immunohistochemistry

Supportive validation

Submitted by

Everest Biotech (provider)

Main image

Experimental details

EB06695 (3.8µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.