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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- R34966 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD32 Antibody / CD32B / FCGR2B
- Antibody type
- Polyclonal
- Description
- This highly specific CD32 antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5 mg/ml in 1X TBS, pH7.3, with 0.5% BSA (US sourced) and 0.02% sodium azide
- Storage
- Aliquot and store the CD32 antibody at -20oC.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Daudi lysate with CD32 antibody at 1ug/ml. Predicted molecular weight: 34-40 kDa depending on the level of glycosylation.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human Daudi lysate with CD32 antibody at 1ug/ml. Predicted molecular weight: 34-40 kDa depending on the level of glycosylation.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IF/ICC staining of fixed and permeabilized human ThP-1 cells with CD32 antibody (green) at 10ug/ml and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human placental tissue with CD32 antibody (left) at 8ugl/ml and without primary Ab (right). Required HIER: steamed antigen retrieval with pH6 citrate buffer; HRP-staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- FACS testing of fixed and permeabilized human K562 cells with CD32 antibody (blue) at 10ug/ml and naive goat Ig (black).
