Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6767 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6767, RRID:AB_1574841
- Product name
- FCGR2B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of FCGR2B.
- Antigen sequence
C-PDALEEPDDQNRI
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Restoration of tolerance in lupus by targeted inhibitory receptor expression.
McGaha TL, Sorrentino B, Ravetch JV
Science (New York, N.Y.) 2005 Jan 28;307(5709):590-3
Science (New York, N.Y.) 2005 Jan 28;307(5709):590-3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FCGR2B polyclonal antibody (Cat # PAB6767) (0.3 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- HEK293 overexpressing FCGR2B and probed with FCGR2B polyclonal antibody (Cat # PAB6767) (mock transfection in first lane), tested by Origene.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FCGR2B polyclonal antibody (Cat # PAB6767)(3.8 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)