Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-63713 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-63713, RRID:AB_960162
- Product name
- Mouse Monoclonal Fc gamma RII/CD32 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. NB100-65338 recognizes the human CD32 antigen, a 40kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII). The antigen mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Clone AT10 blocks the binding of IgG to Fc gamma RII.
- Reactivity
- Human, Canine, Porcine, Simian
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Anti-peptidoglycan antibodies and Fcγ receptors are the key mediators of inflammation in Gram-positive sepsis.
Sun D, Raisley B, Langer M, Iyer JK, Vedham V, Ballard JL, James JA, Metcalf J, Coggeshall KM
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2423-31
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2423-31
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: Fc gamma RII/CD32 Antibody (AT10) [Biotin] [NB100-63713] - Staining of human peripheral blood monocytes with mouse anti human CD32: BIOTIN.