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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
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- Product number
- PA5-18362 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FCGR2B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FCGR2B using FCGR2B Polyclonal Antibody (Product # PA5-18362) (1 µg/mL) in staining of Daudi cell lysate (RIPA buffer, (35 µg protein in RIPA buffer). Detected by chemiluminescence.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of FCGR2B in Human Placenta lysate (RIPA buffer, (35 µg protein in RIPA buffer). Samples were incubated with FCGR2B polyclonal antibody (Product # PA5-18362). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Samples were incubated with FCGR2B polyclonal antibody (Product # PA5-18362) using a dilution of 10 µg/mL for 1 hr followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FCGR2B in human liver. Samples were incubated with FCGR2B polyclonal antibody (Product # PA5-18362) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of FCGR2B in human placenta. Samples were incubated with FCGR2B polyclonal antibody (Product # PA5-18362) using a dilution of 3.75 µg/mL. Formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (PFA fixed) analysis of FCGR2B using FCGR2B Polyclonal Antibody (Product # PA5-18362) (8 µg/mL) in staining of paraffin embedded Human Placenta. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Negative Control for FCGR2B Polyclonal Antibody (Product # PA5-18362), showing immunohistochemical staining of paraffin embedded Human Placenta, with no primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Samples were incubated with FCGR2B polyclonal antibody (Product # PA5-18362) using a dilution of 10 µg/mL for 1hr followed by Alexa Fluor 488 secondary antibody at a dilution of 1 µg/mL. IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
