NB100-1532

antibody from Novus Biologicals

Targeting: FCGR2B CD32, CD32B, FCG2, FCGR2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-1532

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-1532, RRID:AB_10001367

Product name

Goat Polyclonal Fc gamma RII/CD32 Antibody

Antibody type

Polyclonal

References

Submitted references

Evidence for direct contact between the RPA3 subunit of the human replication protein A and single-stranded DNA.

Salas TR, Petruseva I, Lavrik O, Saintomé C
Nucleic acids research 2009 Jan;37(1):38-46

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Restoration of tolerance in lupus by targeted inhibitory receptor expression.

McGaha TL, Sorrentino B, Ravetch JV
Science (New York, N.Y.) 2005 Jan 28;307(5709):590-3

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - HEK293 overexpressing CD32 (RC211982) and probed with EB06695 (mock transfection in first lane).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Fc gamma RII/CD32 Antibody [NB100-1532] - (1ug/ml) staining of Daudi cell lysate (RIPA buffer, (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Fc gamma RII/CD32 Antibody [NB100-1532] - Staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.