Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- BAF1330 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Fc gamma RII/CD32 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Fc gamma RII/CD32 in Western blots. In Western blots, approximately 20% cross-reactivity with recombinant mouse CD32 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P31994
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Cis interaction between sialylated FcγRIIA and the αI-domain of Mac-1 limits antibody-mediated neutrophil recruitment.
Saggu G, Okubo K, Chen Y, Vattepu R, Tsuboi N, Rosetti F, Cullere X, Washburn N, Tahir S, Rosado AM, Holland SM, Anthony RM, Sen M, Zhu C, Mayadas TN
Nature communications 2018 Nov 29;9(1):5058
Nature communications 2018 Nov 29;9(1):5058
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Fc gamma RII/CD32 in Human PBMCs. Fc gamma RII/CD32 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with calcium ionomycin and PMA using Goat Anti-Human Fc gamma RII/CD32 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1330) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to the cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.