Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2558 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal Pentraxin 2/SAP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human and mouse Pentraxin 2/SAP in direct ELISAs and Western blots. In direct ELISA, less 75% cross-reactivity with recombinant rat Pentraxin 2/SAP is observed, and approximately 25% cross-reactivity with recombinant human CRP is observed.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Plasma redox imbalance caused by albumin oxidation promotes lung-predominant NETosis and pulmonary cancer metastasis.
Persistent lung inflammation and fibrosis in serum amyloid P component (APCs-/-) knockout mice.
Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P.
Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P.
Inoue M, Nakashima R, Enomoto M, Koike Y, Zhao X, Yip K, Huang SH, Waldron JN, Ikura M, Liu FF, Bratman SV
Nature communications 2018 Nov 30;9(1):5116
Nature communications 2018 Nov 30;9(1):5116
Persistent lung inflammation and fibrosis in serum amyloid P component (APCs-/-) knockout mice.
Pilling D, Gomer RH
PloS one 2014;9(4):e93730
PloS one 2014;9(4):e93730
Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P.
Pilling D, Roife D, Wang M, Ronkainen SD, Crawford JR, Travis EL, Gomer RH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4035-44
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4035-44
Reduction of bleomycin-induced pulmonary fibrosis by serum amyloid P.
Pilling D, Roife D, Wang M, Ronkainen SD, Crawford JR, Travis EL, Gomer RH
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4035-44
Journal of immunology (Baltimore, Md. : 1950) 2007 Sep 15;179(6):4035-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Pentraxin 2/SAP by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse liver tissue, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Pentraxin 2/SAP at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Pentraxin 2/SAP by Simple WesternTM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for Pentraxin 2/SAP at approximately 36 kDa (as indicated) using 2 µg/mL of Sheep Anti-Human/Mouse Pentraxin 2/SAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2558) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.