LS-C313414

antibody from LSBio

Targeting: APCS MGC88159, PTX2, SAP

ELISA

Antibody data

Submit

Validation data

Product number: LS-C313414 - Provider product page
Provider: LSBio
Product name: APCS / Serum Amyloid P / SAP Antibody (aa21-224) LS-C313414
Antibody type: Polyclonal
Description: Immunogen affinity purified
Reactivity: Mouse
Host: Rabbit
Storage: At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

APCS protein structure - LS-C313414 shown in red.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: WB of APCS / Serum Amyloid P / SAP antibody. All lanes: Anti-Serum Amyloid P at 0.5ug/ml. WB: Recombinant Mouse Serum Amyloid P Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD.

Enhanced validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB902) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25KD. The expected band size for Serum Amyloid P is at 25KD.