RQ4226

antibody from NSJ Bioreagents

Targeting: APCS MGC88159, PTX2, SAP

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4226

Provider

NSJ Bioreagents

Product name

Serum Amyloid P Antibody

Antibody type

Polyclonal

Description

This highly specific Serum Amyloid P antibody is suitable for use in Western blot/Immunohistochemistry/Immunofluorescence/Flow cytometry/ELISA applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

The lyophilized Serum Amyloid P antibody can be stored at 4oC to -20oC. After reconstitution, aliquot and store at -20oC. Avoid repeated freeze/thaws.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human placenta lysate with Serum Amyloid P antibody at 0.5ug/ml. Predicted molecular weight ~25 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human placenta lysate with Serum Amyloid P antibody at 0.5ug/ml. Predicted molecular weight ~25 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HCCT and 2) HCCP cell lysate with Serum Amyloid P antibody at 0.5ug/ml. Predicted molecular weight ~25 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human SiHa cells with Serum Amyloid P antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human rectal cancer tissue with Serum Amyloid P antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human lung cancer tissue with Serum Amyloid P antibody at 1ug/ml. Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human ovarian serous adenocarcinoma tissue with Serum Amyloid P antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with Serum Amyloid P antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer tissue with Serum Amyloid P antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HL60 cells with Serum Amyloid P antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Serum Amyloid P antibody.