PA5-24171

antibody from Invitrogen Antibodies

Targeting: APCS MGC88159, PTX2, SAP

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-24171

Provider

Invitrogen Antibodies

Product name

Serum Amyloid P Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines.

Behrens NE, Lipke PN, Pilling D, Gomer RH, Klotz SA
mBio 2019 Mar 12;10(2)

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIG 1 Binding and phagocytosis of yeasts by human macrophages. (A) SAP binding to C. albicans yeast cells determined by SAP antibody labeling and flow cytometry. (B) SAP binding to yeast cells of C. albicans , S. cerevisiae -EV, the amyloidogenic S. cerevisiae - Als5p WT , and the nonamyloidogenic, S. cerevisiae - Als5p V326N . SAP was offered to yeasts in normal human serum. Each symbol is the average of one of seven independent experiments. (C, left) Binding of fungi to macrophages upon the addition of C. albicans , S. cerevisiae - EV, S. cerevisiae - Als5p WT , or S. cerevisiae - Als5p V326N . (Right) Phagocytosis of the same strains of yeast cells by macrophages. Each symbol is the average from three independent experiments. (D) Effect of SAP on macrophage binding and phagocytosis of C. albicans . (Top left) Binding of yeast cells to control macrophages or macrophages pretreated with SAP. (Top right) Phagocytosis of C. albicans by control macrophages or macrophages pretreated with SAP (30 µg/mL). (Bottom left) Binding of yeast cells in serum-free medium and yeast cells offered SAP in normal human serum to macrophages. (Bottom right) Phagocytosis by macrophages of yeast cells in serum-free medium and yeast cells offered SAP in normal human serum (results of three separate experiments each with 3,200 macrophages counted/variable). Note that the y axis is 10-fold higher for phagocytosis than the binding graphs. Statistical significance was determined by Mann-Whitney U