TA321732

antibody from OriGene

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321732

Provider

OriGene

Product name

Anti-MAP3K5 (Phospho-Ser966) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-MAP3K5 (Phospho-Ser966) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

MAP3K5

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 155 kDa . Positive control: 293 and HepG2 cells treated with UV; 293 cells treated with serum and PMA; Cos7 and Hela cells treated with EGF; Hela cells treated with IFN-?€” lysate. Recommended dilution: 1/ 500-1000. ( Lane1: 293 and HepG2 cells treated with UV; 293 cells treated with serum and PMA; Cos7 and Hela cells treated with EGF; Hela cells treated with IFN-. Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using MAP3K5 (Phospho-Ser966) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC