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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AM06637SU-N - Provider product page
- Provider
- OriGene
- Product name
- ASK1 (MAP3K5) mouse monoclonal antibody, clone 2E4, Ascites
- Antibody type
- Monoclonal
- Description
- ASK1 (MAP3K5) mouse monoclonal antibody, clone 2E4, Ascites
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- MAP3K5
- Isotype
- IgG
- Antibody clone number
- 2E4
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis using MAP3K5 mAb against human MAP3K5 (AA: 922-1108) recombinant protein. (Expected MW is 46.5 kDa)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Red: Control Antigen (100ng) Purple: Antigen (10ng) Green: Antigen (50ng) Blue: Antigen (100ng)
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Hela cells using MAP3K5 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 mouse mAb with DAB staining.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Hela cells using MAP3K5 mouse mAb (green) and negative control (purple).
- Validation comment
- FC
