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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00004217-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004217-M03, RRID:AB_714773
- Product name
- MAP3K5 monoclonal antibody (M03), clone 2D11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant MAP3K5.
- Antigen sequence
SHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKE
LQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFH
LNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDY
TLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFR
NKQT- Isotype
- IgG
- Antibody clone number
- 2D11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MAP3K5 monoclonal antibody (M03), clone 2D11 Western Blot analysis of MAP3K5 expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MAP3K5 expression in transfected 293T cell line by MAP3K5 monoclonal antibody (M03), clone 2D11.Lane 1: MAP3K5 transfected lysate(155 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MAP3K5 is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MAP3K5 on A-431 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
