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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB2279 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2279, RRID:AB_1575585
- Product name
- MAP3K5 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S83 of human MAP3K5.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Requirement of reactive oxygen species-dependent activation of ASK1-p38 MAPK pathway for extracellular ATP-induced apoptosis in macrophage.
SENP1 mediates TNF-induced desumoylation and cytoplasmic translocation of HIPK1 to enhance ASK1-dependent apoptosis.
AIP1 recruits phosphatase PP2A to ASK1 in tumor necrosis factor-induced ASK1-JNK activation.
Noguchi T, Ishii K, Fukutomi H, Naguro I, Matsuzawa A, Takeda K, Ichijo H
The Journal of biological chemistry 2008 Mar 21;283(12):7657-65
The Journal of biological chemistry 2008 Mar 21;283(12):7657-65
SENP1 mediates TNF-induced desumoylation and cytoplasmic translocation of HIPK1 to enhance ASK1-dependent apoptosis.
Li X, Luo Y, Yu L, Lin Y, Luo D, Zhang H, He Y, Kim YO, Kim Y, Tang S, Min W
Cell death and differentiation 2008 Apr;15(4):739-50
Cell death and differentiation 2008 Apr;15(4):739-50
AIP1 recruits phosphatase PP2A to ASK1 in tumor necrosis factor-induced ASK1-JNK activation.
Min W, Lin Y, Tang S, Yu L, Zhang H, Wan T, Luhn T, Fu H, Chen H
Circulation research 2008 Apr 11;102(7):840-8
Circulation research 2008 Apr 11;102(7):840-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of MAP3K5 (arrow) using rabbit MAP3K5 polyclonal antibody (Cat # PAB2279). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP3K5 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human prostate carcinoma tissue reacted with MAP3K5 polyclonal antibody (Cat # PAB2279) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
