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- References [3]
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- Western blot [1]
- ELISA [1]
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- Product number
- PAB9947 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9947, RRID:AB_1706806
- Product name
- MAP3K5 (phospho S83) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphpeptide of MAP3K5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Negative feedback regulation of ASK1 by protein phosphatase 5 (PP5) in response to oxidative stress.
The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1.
Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1.
Morita K, Saitoh M, Tobiume K, Matsuura H, Enomoto S, Nishitoh H, Ichijo H
The EMBO journal 2001 Nov 1;20(21):6028-36
The EMBO journal 2001 Nov 1;20(21):6028-36
The cell cycle-regulatory CDC25A phosphatase inhibits apoptosis signal-regulating kinase 1.
Zou X, Tsutsui T, Ray D, Blomquist JF, Ichijo H, Ucker DS, Kiyokawa H
Molecular and cellular biology 2001 Jul;21(14):4818-28
Molecular and cellular biology 2001 Jul;21(14):4818-28
Molecular mechanisms of the decision between life and death: regulation of apoptosis by apoptosis signal-regulating kinase 1.
Matsuzawa A, Ichijo H
Journal of biochemistry 2001 Jul;130(1):1-8
Journal of biochemistry 2001 Jul;130(1):1-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) shows specificity for phosphorylated human MAP3K5. MAP3K5 (phospho S83) polyclonal antibody was tested by immunoblot against COS-7 cell lysates after transient transfection with (Lane 1) vector only, (Lane 2) recombinant HA-MAP3K5, and (Lane 3) recombinant human HA-MA3K5 where S83 was substituted with an alanine residue.A : anti-HA.B : MAP3K5 (phospho S83) polyclonal antibody blocked with non-phospho peptide.C : MAP3K5 (phospho S83) polyclonal antibody blocked with phospho peptide.A 155 kDa band corresponding to human MAP3K5 is detected.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA results of MAP3K5 (phospho S83) polyclonal antibody (Cat # PAB9947) tested against BSA conjugates of non-phospho and phospho forms of immunizing peptide.Each well was coated with 0.1 mg of conjugate.The starting dilution of antibody was 1 : 1,000 and each point on the X-axis represents a 2-fold dilution.HRP conjugated Goat-anti-Rabbit IgG H&L and TMB substrate were used for detection.
- Validation comment
- ELISA
