PA5-64541

antibody from Invitrogen Antibodies

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64541

Provider

Invitrogen Antibodies

Product name

Phospho-ASK1 (Thr838) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Phospho-ASK1 (Thr838) Polyclonal Antibody detects endogenous levels of ASK1 only when phosphorylated at Thr838.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Chronic-plus-binge alcohol intake induces production of proinflammatory mtDNA-enriched extracellular vesicles and steatohepatitis via ASK1/p38MAPKα-dependent mechanisms.

Ma J, Cao H, Rodrigues RM, Xu M, Ren T, He Y, Hwang S, Feng D, Ren R, Yang P, Liangpunsakul S, Sun J, Gao B
JCI insight 2020 Jul 23;5(14)

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Modification of Cys residues in human thioredoxin-1 by p-benzoquinone causes inhibition of its catalytic activity and activation of the ASK1/p38-MAPK signalling pathway.

Shu N, Hägglund P, Cai H, Hawkins CL, Davies MJ
Redox biology 2020 Jan;29:101400

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASK1 in PMA treated K562 whole cell lysates using a Phospho-ASK1 (Thr838) Polyclonal Antibody (Product # PA5-64541).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 10 Activation of ASK1/p38-MAPK signalling pathway in J774A.1 cells. Cells (1 x 10 6 cells well -1 ) were incubated with different concentrations of BQ (2 and 5 muM) for 15 min, and then cells were harvested and lysed in RIPA buffer containing protease and phosphatase inhibitor cocktail. Samples were analysed by immunoblotting to quantify activation of ASK1 by the phosphorylation of Thr838 (A and B). Cells were incubated with different concentrations of BQ (5 and 10 muM) for 30 min, and then cells were harvested and lysed in RIPA buffer containing a protease and phosphatase inhibitor cocktail. Samples were analysed by immunoblotting (representative images from 3 independent experiments are shown in (A) and (C) and activation of JNK and p38-MAPK measured by the phosphorylation of Thr183/Tyr185 and Thr180/Tyr182, respectively, quantified in panels (B), (D) and (E). The phosphorylated protein levels were normalized to the total of the phosphorylated and unphosphorylated) forms to give the ratio shown in the panels. Levels of beta-actin were also determined as a loading controls to confirm equal protein loading (data not shown). H 2 O 2 (300 muM) was used as a positive control. * p < 0.05, ** p < 0.01 vs. control (CON) samples with no added BQ. Fig. 10