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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- 702278 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASK1 Recombinant Rabbit Monoclonal Antibody (12H12L11)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 12H12L11
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1) A-431 (Lane 2), PC-12 (Lane 3), HT-29 (Lane 4), A549 (Lane 5) and SK-OV-3 (Lane 6). The blots were probed with Anti-ASK1 Recombinant Rabbit Monoclonal Antibody (Product # 702278, 1:200 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 160 kDa band corresponding to ASK1 was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of ASK1 was achieved by transfecting A-431 cells with ASK1 specific siRNA (Silencer® select Product # s198984 and Product # s198985). Western blot analysis (Fig a) was performed using Whole cell extracts from the A-431 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with Anti-ASK1 Recombinant Rabbit Monoclonal Antibody (Product # 702278, 1:200 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). Densitometric analysis of this Western blot is shown in histogram (Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to ASK1.
