ABIN362196

antibody from antibodies-online

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN362196

Provider

antibodies-online

Product name

anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (pSer966) antibody

Antibody type

Polyclonal

Antigen

Peptide sequence around phosphorylation site of pSer966 (S-I-S (p) -L-P) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pSer966

Isotype

IgG

Vial size

100 μL

Concentration

1 mg/mL

Storage

Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

References

Submitted references

Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor.

Zhang W, Zheng S, Storz P, Min W
The Journal of biological chemistry 2005 May 13;280(19):19036-44

---

Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation.

Goldman EH, Chen L, Fu H
The Journal of biological chemistry 2004 Mar 12;279(11):10442-9

---

Negative control of apoptosis signal-regulating kinase 1 through phosphorylation of Ser-1034.

Fujii K, Goldman EH, Park HR, Zhang L, Chen J, Fu H
Oncogene 2004 Jun 24;23(29):5099-104

---

Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 proteins.

Zhang L, Chen J, Fu H
Proceedings of the National Academy of Sciences of the United States of America 1999 Jul 20;96(15):8511-5