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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
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- Product number
- ABIN499347 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody
- Antibody type
- Polyclonal
- Antigen
- Human ASK1 Peptide (AP30086CP-N)
- Description
- Affinity chromatography purified via peptide column
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Molecular cloning and characterization of the mouse apoptosis signal-regulating kinase 1.
Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.
Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.
Tobiume K, Inage T, Takeda K, Enomoto S, Miyazono K, Ichijo H
Biochemical and biophysical research communications 1997 Oct 29;239(3):905-10
Biochemical and biophysical research communications 1997 Oct 29;239(3):905-10
Induction of apoptosis by ASK1, a mammalian MAPKKK that activates SAPK/JNK and p38 signaling pathways.
Ichijo H, Nishida E, Irie K, ten Dijke P, Saitoh M, Moriguchi T, Takagi M, Matsumoto K, Miyazono K, Gotoh Y
Science (New York, N.Y.) 1997 Jan 3;275(5296):90-4
Science (New York, N.Y.) 1997 Jan 3;275(5296):90-4
Molecular cloning and characterization of a novel protein kinase with a catalytic domain homologous to mitogen-activated protein kinase kinase kinase.
Wang XS, Diener K, Jannuzzi D, Trollinger D, Tan TH, Lichenstein H, Zukowski M, Yao Z
The Journal of biological chemistry 1996 Dec 6;271(49):31607-11
The Journal of biological chemistry 1996 Dec 6;271(49):31607-11
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of ASK1 in SW1353 cell lysate with AP30086PU-N ASK1 antibody at (A) 0.5, (B) 1, and (C) 2 ?g/ml.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunofluorescence of ASK1 in 3T3 cells with AP30086PU-N ASK1 antibody at 20 ?g/ml.
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Immunocytochemistry of ASK1 in A431 cells with AP30086PU-N ASK1 antibody at 2 ?g/ml.
