MA5-42430

antibody from Invitrogen Antibodies

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot

Immunohistochemistry

Antibody data

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Validation data

Product number: MA5-42430 - Provider product page
Provider: Invitrogen Antibodies
Product name: ASK1 Recombinant Rabbit Monoclonal Antibody (SU36-06)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Positive Control: 293 cell lysates, A549, Hela, MCF-7, human lung carcinoma tissue, human pancreas tissue, mouse pancreas tissue, human breastcarcinoma tissue. Subcellular Location: Endoplasmic reticulum, Cytoplasm.
Reactivity: Human, Mouse
Host: Rabbit
Isotype: IgG
Antibody clone number: SU36-06
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

MAP3K5 protein structure - MA5-42430 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of ASK1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ASK1 Recombinant Monoclonal Antibody (Product # MA5-42430) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of ASK1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ASK1 Recombinant Monoclonal Antibody (Product # MA5-42430) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of of ASK1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with ASK1 Recombinant Monoclonal Antibody (Product # MA5-42430) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).