Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-15861 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASK1 Monoclonal Antibody (2E4)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15861 targets MAP3K5 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples. The MA5-15861 immunogen is purified recombinant fragment of human MAP3K5 expressed in E. Coli. . MA5-15861 detects MAP3K5 which has a predicted molecular weight of approximately 55kDa.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E4
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Syndecan-1 (CD138) Suppresses Apoptosis in Multiple Myeloma by Activating IGF1 Receptor: Prevention by SynstatinIGF1R Inhibits Tumor Growth.
Beauvais DM, Jung O, Yang Y, Sanderson RD, Rapraeger AC
Cancer research 2016 Sep 1;76(17):4981-93
Cancer research 2016 Sep 1;76(17):4981-93
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MAP3K5 using a MAP3K5 monoclonal antibody (Product # MA5-15861) against a human MAP3K5 (AA: 922-1108) recombinant protein.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using MAP3K5 monoclonal antibody (Product # MA5-15861) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and prostate tissues (right) using MAP3K5 monoclonal antibody (Product # MA5-15861) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using MAP3K5 monoclonal antibody (Product # MA5-15861) (green) and negative control (purple).
