PA5-28692

antibody from Invitrogen Antibodies

Targeting: MAP3K5 ASK1, MAPKKK5, MEKK5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28692

Provider

Invitrogen Antibodies

Product name

ASK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLaS3, mouse lung, MAP3K5-transfected 293T.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASK1 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ASK1 Polyclonal Antibody (Product # PA5-28692) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using ASK1 Polyclonal Antibody (Product # PA5-28692). Sample (30 µg whole cell lysate). A: HeLa S3.5% SDS PAGE. ASK1 Polyclonal Antibody (Product # PA5-28692) diluted at 1:1,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASK1 Polyclonal Antibody detects MAP3K5 protein by Western blot analysis. A. 50 µg mouse lung lysate/extract.5 % SDS-PAGE. ASK1 Polyclonal Antibody (Product # PA5-28692) dilution: 1:1,000.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ASK1 (Product # PA5-28692) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.