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Learn11935-1-AP
antibody from Invitrogen Antibodies
Targeting: CCNE2
CYCE2
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11935-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cyclin E2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MSRRSSRLQ AKQQPQPSQT ESPQEAQIIQ AKKRKTTQDV KKRREEVTKK HQYEIRNCWP PVLSGGISPC IIIETPHKEI GTSDFSRFTN YRFKNLFINP SPLPDLSWGC SKEVWLNMLK KESRYVHDKH FEVLHSDLEP QMRSILLDWL LEVCEVYTLH RETFYLAQDF FDRFMLTQKD INKNMLQLIG ITSLFIASKL EEIYAPKLQE FAYVTDGACS EEDILRMELI ILKALKWELC PVTIISWLNL FLQVDALKDA PKVLLPQYSQ ETFIQIAQLL DLCILAIDSL EFQYRILTAA ALCHFTSIEV VKKASGLEWD SISECVDWMV PFVNVVKSTS PVKLKTFKKI PMEDRHNIQT HTNYLAMLCM ISSHV (1-374 aa encoded by BC020729)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IGG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 11935-1-AP ( Cyclin E2 antibody at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis tissue slide using 11935-1-AP ( Cyclin E2 antibody at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were stained with 0.2ug Cyclin E2 antibody (11935-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug.
