MA5-42447

antibody from Invitrogen Antibodies

Targeting: CCNE2 CYCE2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-42447

Provider

Invitrogen Antibodies

Product name

Cyclin E2 Recombinant Rabbit Monoclonal Antibody (SD2035)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

Positive Control: NIH/3T3, Hela, MCF-7, human breast carcinoma tissue. Subcellular Location: Nucleus.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

SD2035

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of Cyclin E2 in NIH/3T3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin E2 Recombinant Monoclonal Antibody (Product # MA5-42447) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of Cyclin E2 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Cyclin E2 Recombinant Monoclonal Antibody (Product # MA5-42447) at a dilution of 1:500 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of Cyclin E2 in HeLa cells using Cyclin E2 Recombinant Monoclonal Antibody (Product # MA5-42447) at 1:50 (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1:1,000 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).