Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B1441 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ CAT / Catalase Antibody LS-B1441
- Antibody type
- Polyclonal
- Description
- Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
- Reactivity
- Bovine
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-Catalase Polyclonal Antibody-Western blot. Rabbit anti-Catalase antibody was used to detect Catalase under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified Catalase contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of anti-Catalase antibody and detection was using Dylight 488 conjugated Donkey anti-rabbit (1:10K 1.5 hr RT in MB-070) imaged on the BioRad VersaDoc System.